mouse p-cadherin antibody  (Bio-Techne corporation)

Journal: iScience

Article Title: Selective deletion of Slc2a1 from the RPE reveals that rods but not cones depend on glucose transport across the outer blood-retinal barrier

doi: 10.1016/j.isci.2025.114450

Figure Lengend Snippet: RPE lacking GLUT1 remain viable (A) Tiled confocal image of a flattened RPE eyecup from an RPE-iΔGlut1 mouse at 62 weeks PT, immunostained for P-cadherin (white). Dashed circles denote the following concentric zones: (1) A central circular zone with a radius of 0.85 mm from the optic nerve center, (2) a central annular zone of 0.85–1.7 mm radial distance, and (3) the outer annular zone which encompasses 1.7–2.6 mm radial distance from the optic nerve. Scale bar denotes 1 mm. (B) Eyecups of control and RPE-iΔGlut1 eyes were immunolabeled with anti-P-cadherin and cross-sectional area was estimated from 3 to 4 confocal z -projections of 0.025 mm 2 fields of view from each mouse within zones 2 and 3. Data are from n = 3 control and n = 4 RPE-iΔGlut1 mice for zone 2, and n = 3 control and n = 3 RPE-iΔGlut1 mice for zone 3. All mice were 62–65 weeks PT. Bars denote mean (±SEM); ns, not significantly different; Student’s t test. (C) Example z-stacks showing P-cadherin immunolabeling (green) with DAPI counterstain (white) from a control and RPE-iΔGlut1 mouse at 62 weeks PT. Scale bars denote 20 μm. (D) Sagittal cryosections from a control and RPE-iΔGlut1 mouse at 62 weeks PT immunostained for MCT3 (red) with phalloidin/f-actin (white) and DAPI counterstains (cyan). Scale bars denote 20 μm. RPE, retinal pigment epithelium; OS/IS, outer and inner segments.

Article Snippet: Goat anti - p -cadherin , R & D Systems , Cat#AF761; RRID: AB_355581.

Techniques: Control, Immunolabeling

Journal: iScience

Article Title: Selective deletion of Slc2a1 from the RPE reveals that rods but not cones depend on glucose transport across the outer blood-retinal barrier

doi: 10.1016/j.isci.2025.114450

Figure Lengend Snippet: Persistence of sub-retinal microglia and gliosis in retinas of RPE-iΔGlut1 mice (A) Tiled confocal z-stack of a flattened RPE/eyecup from an RPE-iΔGlut1 mouse at 6 weeks PT, immunolabeled with an antibody to ionized calcium-binding adaptor molecule 1 (Iba-1, white). (B) Top, enlarged view of boxed region in (A), showing Iba-1 (red) along with anti-P-cadherin immunolabeling (green). Bottom, same sized field of view from another RPE/eyecup flat mount taken from an RPE-iΔGlut1 mouse at 62 weeks PT (Iba-1, red; P-cadherin, green). Scale bars denote 50 μm for all panels. (C) Immunolabeling with anti-Iba-1 (red) and Galectin-3 (Gal-3, green) in sagittal cryosections from an RPE-iΔGlut1 mouse at 10 weeks (top) and 62 weeks PT (bottom). Scale bars denote 20 μm for all panels. (D) Western blot showing Gal-3 immunopositive bands in control and RPE-iΔGlut1 mouse retinal lysates at 10 weeks PT. Retina lysates from control ( n = 4) and RPE-iΔGlut1 ( n = 4) mice were used to quantify Gal-3 relative to loading control (D, right). Bars denote mean (±SD). p < 0.05, Student’s t test. (E) Western blot showing Gal-3 immunopositive bands in control and RPE-iΔGlut1 mouse retinal lysates at 62 weeks PT. Retinal lysates from control ( n = 4) and RPE-iΔGlut1 ( n = 4) mice were used to quantify Gal-3 relative to loading control (right). Bars denote mean (±SD). p < 0.05 (∗), Student’s t test. (F) Sagittal cryosections from 10 weeks PT control (left) and RPE-iΔGlut1 mice at 10 weeks PT (middle) and 62 weeks PT (right) immunolabled with an anti-GFAP antibody. Arrows point to the OLM. Scale bars denote 20 μm for all panels. (G) GFAP immunolabeled bands in western blots of retinal lysates from control and RPE-iΔGlut1 mice at 10 weeks PT. Right: quantification of GFAP relative to loading control from control ( n = 4) and RPE-iΔGlut1 ( n = 3) mice. Bars denote mean (±SD). p < 0.05 (∗), Student’s t test. (H) GFAP immunolabeled bands in western blots of retinal lysates from control and RPE-iΔGlut1 mice at 62 weeks PT. Right: quantification of GFAP relative to loading control from control ( n = 5) and RPE-iΔGlut1 ( n = 5) mice. Bars denote mean (±SD). p < 0.01 (∗∗), Student’s t test.

Article Snippet: Goat anti - p -cadherin , R & D Systems , Cat#AF761; RRID: AB_355581.

Techniques: Immunolabeling, Binding Assay, Western Blot, Control